Chemistry of life (CE10/MSM-ME6)15 ECTS
(englische Bezeichnung: Chemistry of life)
(Prüfungsordnungsmodul: Wahlpflichtmodul Molecular Science)

Lehrveranstaltungen:

• A. Chemistry of Biomolecules and Cellular Functions (2 SWS/VORL)
  • Chemistry of Biomolecules and Cellular Functions (WS 2017/2018)
    (Vorlesung, Andriy Mokhir, Do, 10:00 - 11:30, A00.14)
• B. Cell Signaling and chemistry of oxidative stress (2 SWS/VORL)
  • Cell Signaling and chemistry of oxidative stress (SS 2018)
    (Vorlesung, 2 SWS, Ivana Ivanovic-Burmazovic, Do; block seminar 23.4 - 27.4.2018, 14-17 o´clock, place: A00.12)
• C. Seminar: Experimental Techniques and Selected Topics in Chemical Biology of Diseases (1SWS/SEM)
  • Experimental Techniques and Selected Topics in Chemical Biology of Diseases (part1) (WS 2017/2018)
    (Seminar, 1 SWS, Milos Filipovic et al., second part of the seminar in the summer term, part A presumably 19.02.-02.03.2018, part B presumably 07.05.-11.05.2018)
  • Experimental Techniques and Selected Topics in Chemical Biology of Diseases (part2) (SS 2018)
    (Seminar, 1 SWS, Ivana Ivanovic-Burmazovic, Do, 11:45 - 12:30, A 1.42 Egerlandstr.1; second half of the seminar in SS14)
• D . Practical session (10SWS/PRA)

  Attendance in lab course is compulsory!

  • Chemistry of Life - Practical session (WS 2017/2018)
    (Praktikum, Ivana Ivanovic-Burmazovic et al., registration via StudON, practical session presumably: part A1 (Filipovic): 19.02.2018-02.03.2018, part A2 (Filipovic): 07.05.-11.05.2018, part B1 and B2 (Mokhir) on appointment)
  • Chemistry of Life - Practical session (SS 2018)
    (Praktikum, Ivana Ivanovic-Burmazovic et al., this second part of the lab course will take place in may)

Inhalt:

A.

• Relation between the 3D protein structure and their function, including discussion of state-of-the-art methodology

• Biochemistry of biological membranes (chemistry of carbohydrates, lipids an protein channels)

• Chemical biology of nucleic acids as therapeutic targets

• Protein-nucleic acid interactions

• Cell as self-sustainable and bio-functional confined space

• Nucleosides and Nucleotides

• Chemical and biological synthesis of nucleic acids

• DNA and RNA structure

• Nucleic acids in biotechnology

• Spectroscopic and structural methods applied in studies of nucleic acids

• Nucleic acids and their analogues as drugs

B.

• Bioinorganic chemistry of electron transfer

• Thermodynamics and kinetics of mitochondrial processes

• Biochemistry of free radical generation and removal

• Monitoring oxidative stress in living systems

• Interplay between oxidative stress and cell signaling pathways: Inflammation, Neuropathie and Cancer as model systems

• Redox drugs

C. Seminar: Preparation for practical session with an accent on the methodological approach and medical aspects

D.
D1: Structure and function of proteins: denaturation/renaturation, protein-ligand interactions (UV-Vis, fluorescence and EPR spectroscopy, amperometric analysis of small-molecule interactions)
D2: Kinetics of SOD and Catalase activity of natural enzymes and their synthetic mimics (direct stopped-flow methods vs. indirect assays)
D3: Cell preparation for fluorescence microscopy (staining of cell compartments and detection of oxidative stress)
D4: Protein purification and analysis (cell extract preparation, 2D-electrophoresis of total cell extract, trypsin digestion, HPLC separation and ESI-MS-detection)
D5: Synthesis of a representative nucleic acid analogue; its identification (MALDI-TOF MS), purification (HPLC), quantification (UV-visible spectroscopy), study of binding to a target nucleic acid (fluorescence spectroscopy, melting profile measurement).
D6: As a DEMO-Experiment: testing inhibitors in cellular cultures, monitoring inhibition by using flow cytometry and RT PCR

Lernziele und Kompetenzen:

Students

• are provided with the up-to-date practical and operative know-how suitable for future scientific and/or applied work in research institutes, pharmaceutical/food industry, medical care laboratories, bio-technological, bio-analytical and environmental branches

• get an advanced theoretical background and an overview of emerging trends in life sciences (chemistry, biology and medicine)

• look at living systems through the lens of basic chemical principles

• are prepared to work in interdisciplinary environment and participate in national and international development of forefront fields such as translational medicine.

Literatur:

Selected chapters from:

• Voet & Voet, Biochemistry, Wiley & Sons;

• Barry Halliwell & John M. C. Gutteridge, Free Radicals in Biology and medicine, Oxford

• I. M. Rosenberg, Protein Analysis and Purification, Birkhäuser

• Bertini, Gray, Stiefe, Valentine, Biological Inorganic Chemistry, Structure & Reactivity, University Science Books

• F. Marks, U. Klingmüller, K. Müller-Decker, Cellular Signal Processing: An Introduction to the Molecular Mechanisms of Signal Transduction, Taylor & Francis

Bemerkung:

Module compatibility: M.Sc. Molecular (Life) Science (Mandatory elective module/ Wahlpflichtmodul)/ M.Sc. Chemistry (Elective Module/Wahlmodul)

Organisatorisches:

Module frequency: Annually, starting only in winter term;
the module can only be taken as a whole

Verwendbarkeit des Moduls / Einpassung in den Musterstudienplan:

1. Molecular Science (Master of Science)
   (Po-Vers. 2013 | NatFak | Molecular Science (Master of Science) | Wahlpflichtmodul Molecular Science)

Studien-/Prüfungsleistungen: