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Orientierungsmodul Biochemie III: Introduction to Sequence Analysis (BCMA III)7.5 ECTS (englische Bezeichnung: Orientierungsmodul Biochemie III: Einführung in die Sequenzanalyse)
Modulverantwortliche/r: Uwe Sonnewald Lehrende:
Uwe Sonnewald, Sophia Sonnewald, José María Corral García
Startsemester: |
SS 2020 | Dauer: |
1 Semester | Turnus: |
jährlich (SS) |
Präsenzzeit: |
120 Std. | Eigenstudium: |
105 Std. | Sprache: |
Deutsch |
Lehrveranstaltungen:
Empfohlene Voraussetzungen:
keine
Inhalt:
- Introduction to bioinformatics, sequence analysis and sequence formats.
Primer design, sequence alignments and sequence assembly.
Sequence databases, similarity search (BLAST) and DNA barcoding.
Experimental design for omics projects.
Gene analysis and alternative splicing.
Regulation of gene expression.
Promoter analysis and prediction of TBFs and microRNA targets.
Genetic markers (SNPs, microsatellites and INDELs).
Next Generation Sequencing (NGS) data processing.
Genome mapping and annotation.
Transcriptome analysis methods (Microarray and RNA-seq).
Epigenetic local and high-throughput analysis.
Molecular diagnosis and personalized medicine.
SNPs analysis for disease human prediction.
Mutagenesis and directed evolution.
Strategical workflow and establishment of analytical bioinformatics pipelines.
Lernziele und Kompetenzen:
Students able to:
use databases, online tools and software for sequence analysis.
practice with similarity search of sequences (BLAST).
predict and categorize sequence features as gene-structures, reading frames, active sites, post-translational modification sites, distribution of exons and introns, and regulatory elements.
discover genetic markers by identification of sequence variations as SNPs, INDELs or microsatellites.
predict different levels of mRNA and protein structures.
analyze and compute Next Generation Sequencing datasets.
relate and integrate genomic and transcriptomic data with phenotype.
generate analytical workflows combining different applications and formats.
practice with different sequence datasets and develop an own hypothetical project of sequence analysis with bioinformatics tools.
prepare a presentation and a written scientific report describing and discussing the results obtained in the practical project with own data.
Literatur:
keine
Studien-/Prüfungsleistungen:
BCMA III: Introduction to Sequence Analysis (Prüfungsnummer: 22802)
- Prüfungsleistung, mehrteilige Prüfung, benotet, 7.5 ECTS
- Anteil an der Berechnung der Modulnote: 100.0 %
- weitere Erläuterungen:
PL: oral examination (30 min.)
PL: Protokol (approx. 3-5 pages)
- Erstablegung: SS 2020
1. Prüfer: | José María Corral García |
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UnivIS ist ein Produkt der Config eG, Buckenhof |
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