relevant culture systems (T-Flask and bioreactor; interactions with the cells, products, and nutrients)

production of antibodies with hybridoma cells, insect cells and baculoviruses (biopesticides, recombinant proteins)

immobilization (procedure, materials, xenogeneic transplants, immune protection, maximizing the yield of recombinant proteins)

establishment of cell lines

characterization of cell cultures (microscopy, trypan blue, MTT, resazurin, EZ4U in CFDA-SE, PJ, FACS, MACS)

typical metabolic pathways in cell cultures

needs of cell cultures (nutrients, pO2, adherence, shear stress, buffer systems, pH)

medically relevant applications (liver replacement systems, cancer therapy, artificial skin)

toxicity and toxicity tests

stem cell procedures and cell differentiation