Basic Integrated Laboratory Course (iI-BI Lab)10 ECTS
(englische Bezeichnung: Basic Integrated Laboratory Course)

Lehrveranstaltungen:

• • Practical Integrated Laboratory Course - Basic Immunology (with Tutorial)
    (Praktikum, 12 SWS, Lars Nitschke et al., 9:00 - 18:00, SR BTE)

Inhalt:

Course:

• identification of the organs of the immune system in mice

• isolation and purification of immune cells from laboratory mice

• detailed characterization of immune cells by flow cytometry

• in vitro stimulation of immune cells

• quantification of humoral and cellular immune responses in vitro and in vivo

• histological analysis of immune organs

• DNA and RNA based techniques for quantifications of immune responses

• genetic engineering of mice
  

Tutorials:
In small groups (e.g. 5 students) the students will design experimental setups for the generation and the analysis of mice with defined mutations in genes of the immune system

Lernziele und Kompetenzen:

Students are able to

• isolate immune cells from laboratory animals

• perform cellular characterizations of immune cells by flow cytometry including detailed quantitative analyses

• characterize humoral and cellular immune responses against model antigens

• perform cell culture experiments with immune cells

• plan and perform basic techniques in molecular biology for the molecular analysis of innate and adaptive immune responses

• understand, summarize and apply major laboratory techniques in immunology

• independently develop experimental strategies to test a scientific hypothesis

Literatur:

Janeway's Immunobiology (9th Edition)

Verwendbarkeit des Moduls / Einpassung in den Musterstudienplan:
Das Modul ist im Kontext der folgenden Studienfächer/Vertiefungsrichtungen verwendbar:

1. Integrated Immunology (Master of Science)
   (Po-Vers. 2018w | Gesamtkonto | Basic Integrated Laboratory Course)

Studien-/Prüfungsleistungen:

Praktikumsbericht zu Basic Immunology Laboratory Course (Prüfungsnummer: 78501)

(englischer Titel: Laboratory report: Basic immunology)

Untertitel: Basic Integrated Laboratory Course

Studienleistung, schriftlich, unbenotet, 8 ECTS

Prüfungssprache: Englisch

Erstablegung: WS 2021/2022, 1. Wdh.: SS 2022

1. Prüfer:

Lars Nitschke

Referat zu Basic Immunology Laboratory Course (Prüfungsnummer: 78502)

(englischer Titel: Presentation: Basic immunology)

Studienleistung, Referat, unbenotet, 2 ECTS

Erstablegung: WS 2021/2022, 1. Wdh.: SS 2022

1. Prüfer:

Lars Nitschke