Orientierungsmodul Entwicklungsbiologie 3: Visualization of Gene Regulation during Development (OMA-Ebio 3)7.5 ECTS
(englische Bezeichnung: Orientational Module Developmental Biology 3: Visualization of Gene Regulation during Development)
(Prüfungsordnungsmodul: Entwicklungsbiologie 3: Visualization of Gene Regulation during Development)

Lehrveranstaltungen:

• • Orientierungsmodul Entwicklungsbiologie 3: Visualization of Gene Regulation during Development
    (Übung, 8 SWS, Ezzat El-Sherif, 9:00 - 17:00, CIP Biologie)

Empfohlene Voraussetzungen:

keine

Inhalt:

Seminar talks will cover basic principles of transcription in eukaryotes and different methods to investigate them, with more focus on imaging-based techniques. DNA/RNA labelling techniques and confocal and super-resolution microscopy will be discussed, emphasising on how they are applied to the study of gene regulation during development. Basics of computational methods to analyse microscopy data using Matlab and ImageJ will be covered.
In the laboratory course, we start with applying single molecule FISH to label mRNA and enhancer RNA in fixed Drosophila embryos. Students will then use confocal and STED microscopy to visualize transcription products, then use Matlab and ImageJ to process the data they collected. The final phase is the data analysis, where students will investigate the correlation between enhancer, gene, and enhancer RNA activities and their co-localization in the nucleus, and how this sheds light on important events in the transcription cycle

Lernziele und Kompetenzen:

Students will

• have a basic understanding of principles of gene regulation in eukaryotes.

• be familiar with experiments to investigate gene regulation, especially imaging-based techniques.

• understand and apply RNA/DNA labeling techniques in fixed embryos (single-molecule FISH).

• understand and apply confocal and super-resolution microscopy (STED and STORM).

-understand and apply Image processing techniques for analyzing microscopy data (using Matlab and ImageJ).

Literatur:

Schulwissen der Mathematik im Umfang von Abschnitt 2 bis 15 des Buches „Startwissen Mathematik und Statistik“ von Harris, Taylor, Taylor (Spektrum Verlag 2007)

Verwendbarkeit des Moduls / Einpassung in den Musterstudienplan:

1. Zell- und Molekularbiologie (Master of Science)
   (Po-Vers. 2019w | NatFak | Zell- und Molekularbiologie (Master of Science) | Orientierungsmodule | Biologische Orientierungsmodule | Entwicklungsbiologie 3: Visualization of Gene Regulation during Development)

Studien-/Prüfungsleistungen:

Entwicklungsbiologie 3: Computersimulationen embryonaler Musterbildungsprozesse (Prüfungsnummer: 82201)

Prüfungsleistung, mehrteilige Prüfung, benotet, 7.5 ECTS

Anteil an der Berechnung der Modulnote: 100.0 %

weitere Erläuterungen:
PL: schriftliche Klausur 60 Min. SL: mündliches Referat 30 Min. PL: schriftliche Protokolle

Erstablegung: WS 2022/2023, 1. Wdh.: SS 2023

1. Prüfer:

Ezzat El-Sherif