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Integrated Life Sciences: Biology, Biomathematics, Biophysics (Master of Science) >>

ILS-MA-B5: Developmental Biology 3: Visualization of Gene Regulation during Development (ILS-MA-B5)7.5 ECTS
(englische Bezeichnung: ILS-MA-B5: Entwicklungsbiologie 3: Visualisierung der Genregulation bei der Entwicklung)
(Prüfungsordnungsmodul: Developmental Biology 3: Visualization of Gene Regulation during Development)

Modulverantwortliche/r: Ezzat El-Sherif
Lehrende: Ezzat El-Sherif


Startsemester: WS 2022/2023Dauer: 1 SemesterTurnus: jährlich (WS)
Präsenzzeit: 120 Std.Eigenstudium: 105 Std.Sprache: Englisch

Lehrveranstaltungen:


Empfohlene Voraussetzungen:

none

Inhalt:

Seminar talks will cover basic principles of transcription in eukaryotes and different methods to investigate them, with more focus on imaging-based techniques. DNA/RNA labelling techniques and confocal and super-resolution microscopy will be discussed, emphasising on how they are applied to the study of gene regulation during development. Basics of computational methods to analyse microscopy data using Matlab and ImageJ will be covered.
In the laboratory course, we start with applying single molecule FISH to label mRNA and enhancer RNA in fixed Drosophila embryos. Students will then use confocal and STED microscopy to visualize transcription products, then use Matlab and ImageJ to process the data they collected. The final phase is the data analysis, where students will investigate the correlation between enhancer, gene, and enhancer RNA activities and their co-localization in the nucleus, and how this sheds light on important events in the transcription cycle.

Lernziele und Kompetenzen:

Students will

  • have a basic understanding of principles of gene regulation in eukaryotes.

  • be familiar with experiments to investigate gene regulation, especially imaging-based techniques.

  • understand and apply RNA/DNA labeling techniques in fixed embryos (single-molecule FISH).

  • understand and apply confocal and super-resolution microscopy (STED and STORM).

  • understand and apply Image processing techniques for analyzing microscopy data (using Matlab and ImageJ).

Literatur:

Introductory review articles will be provided electronically


Verwendbarkeit des Moduls / Einpassung in den Musterstudienplan:

  1. Integrated Life Sciences: Biology, Biomathematics, Biophysics (Master of Science)
    (Po-Vers. 2019w | NatFak | Integrated Life Sciences: Biology, Biomathematics, Biophysics (Master of Science) | Module Groups | MG1: Mathematical Modelling and Systems Biology | Mandatory Elective Modules Group 1 | Developmental Biology 3: Visualization of Gene Regulation during Development)

Studien-/Prüfungsleistungen:

Portfolio exam: Developmental Biology 3: Computer Simulations of Embryonal Pattern Formation (Prüfungsnummer: 82211)

(englischer Titel: Portfolio exam: Developmental Biology 3: Computer Simulations of Embryonal Pattern Formation)

Prüfungsleistung, mehrteilige Prüfung, benotet, 7.5 ECTS
Anteil an der Berechnung der Modulnote: 100.0 %
weitere Erläuterungen:
PL: written examination of 45 min. PL: protocol of 10-15 pages SL: seminar talk of ca. 30 min. (ungraded task)

Erstablegung: WS 2022/2023
1. Prüfer: Ezzat El-Sherif

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