| Spray Drying of Proteins Spray drying of therapeutic proteins has two uses. First, to produce protein loaded particles suitable for pulmonary application. Secondly, to stabilise protein bulk as an alternative to freeze drying. In Erlangen we examine the effect of process variables on the stability and morphology of spray dried protein particles. Currently, we examine a number of aspects. Spray drying of immunoglobulins is being optimised to maintain maximal monomer content and maximum powder yield. Chances in the folding of proteins during atomisation and drying is being examined using FT-IR and CD on model alpha-helix, beta-fold and unordered model proteins. The drying rate and behaviour of single droplets of proteins and sugars with surfactants are being investigated using a single droplet drying levitator. Formulation of spray-dried protein powder is examined in detail. | Projektleitung:
Prof. i. R. Dr. Geoffrey Lee, Apoth.
Beteiligte:
Peter Lassner, Apoth., Michael Maury, Apoth., Alexander Mauerer, Apoth., Heiko Schiffter, Apoth., Jürgen Bögelein, Apoth.
Stichwörter:
spray drying; protein; stability; carbohydrate; folding; stability, single droplet drying
Beginn: 1.12.1996
Förderer:
DFG (Projekt Le 626/7-1)
Abbott GmbH & Co KG, Ludwigshafen
Niro A/S, Soeborg (Denmark)
| | Publikationen |
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| Adler, Michael ; Lee, Geoffrey: Stability and Surface Activity of Lactate Dehydrogenase in Spray-Dried Trehalose. In: Journal of Pharmaceutical Sciences 88 (1999), Nr. 2, S. 199-208 | | Adler, Michael ; Unger, Michael ; Lee, Geoffrey: Surface Composition of Spray-Dried Particles of Bovine Albumin/Trehalose/Surfactant. In: Pharmaceutical Research 17 (2000), Nr. 7, S. 863-870 | | Lee, Geoffrey: Spray-Drying of Proteins. In: Carpenter, John ; Manning, Mark (Hrsg.) : Rational Design of Stable Protein Formulation. 13. Aufl. New York : Kluwer Academic/Plenum Publishers, 2002, S. 135-158. - ISBN 0-306-46741-0 |
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